1.8 mT不同频率正弦交变电磁场对青年大鼠骨密度及骨形态计量的影响比较

Effects of 1.8 mT sinusoidal electromagnetic fields of different frequencies on bone mineral density and bone histomorphometry of young rats

摘要:

比较不同频率的正弦交变电磁场对SD青年大鼠骨密度及骨形态计量指标的影响，筛选可有效提升大鼠骨密度的频率参数。将32只8周龄SD雌性大鼠随机分为4组：对照组、15 Hz组、30 Hz组、45 Hz组；除对照组外，实验组大鼠每天都给予相应频率的1.8 mT正弦交变电磁场干预，干预时间为90 min。磁场干预8周后，双能X射线骨密度仪检测大鼠全身骨密度、右侧股骨骨密度和椎骨骨密度，ELISA分析血清中骨形成与骨吸收生化指标的含量，右侧胫骨进行荧光间距测量与骨形态计量分析。相比于对照组，15 Hz组、45 Hz组大鼠的全身骨密度、股骨骨密度、椎骨骨密度均明显升高 (P<0.05)，血清中骨钙素与骨保护素含量也显著提升 (P<0.05)；实验组大鼠的胫骨双荧光间距与骨组织静态参数均高于对照组 (P<0.05)。结果表明，15 Hz、45 Hz正弦交变电磁场可有效提升青年大鼠的骨密度，从而可预防骨质疏松的发生。

Abstract:

To study the effects of different frequency sinusoidal electromagnetic fields on the bone mineral density and bone histomorphometry of SD young rats, and to screen suitable electromagnetic field frequency. In total 32 female SD rats of 8 weeks old were randomly divided into 4 groups: control group, 15 Hz group, 30 Hz group and 45 Hz group. Except for the control group, rats in experimental groups were treated with corresponding frequency of 1.8 mT sinusoidal electromagnetic field 90 minutes every day. Rats were measured for bone mineral density after 8 weeks by dual energy X-ray absorptiometry. Rats’ femur and vertebral bones were measured by analyzing the static and dynamic forms on the right tibia bone for morphometrics. Rat serum was measured to estimate the index of bone formation and bone resorption. Bone mineral density of rats from 15 Hz group and 45 Hz group was higher than that of the control group (P<0.05). Serum osteocalcin level of rats from 15 Hz group and 45 Hz group was higher than that of the control group (P<0.05). Double fluorescence spacing and static parameters of bone tissue in experimental group rat tibia were higher than that of the control group (P<0.05). Our findings imply that 15 Hz and 45 Hz sinusoidal electromagnetic fields can effectively increase bone mineral density in young rats for preventing osteoporosis.