1 Shanghai Tenth Peoples Hospital, Department of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China 在期刊界中查找 在百度中查找 在本站中查找
1 Shanghai Tenth Peoples Hospital, Department of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China 在期刊界中查找 在百度中查找 在本站中查找
1 Shanghai Tenth Peoples Hospital, Department of Bioinformatics, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China 在期刊界中查找 在百度中查找 在本站中查找
CRISPR-based genome editing has been widely implemented in various cell types. In-silico single guide RNA (sgRNA) design is a key step for successful gene editing using CRISPR system. Continuing efforts are made to refine in-silico sgRNA design with high on-target efficacy and reduced off-target effects. In this paper, we summarize the present sgRNA design tools, and show that efficient in-silico models can be built that integrate current heterogeneous genome-editing data to derive unbiased sgRNA design rules and identify key features for improving sgRNA design. Our review shows that systematic comparisons and evaluation of on-target and off-target effects of sgRNA will allow more precise genome editing and gene therapies using the CRISPR system.