降钙素原荧光免疫层析方法的建立

Detection of procalcitonin based on fluorescence immune chromatography

摘要:

降钙素原 (Procalcitonin，PCT) 是降钙素的前体物质，与人体细菌感染情况严重程度相关。通过PCT抗体与量子点偶联，自制试纸条，开发一种高灵敏度、快速便捷的PCT检测产品，对其初步应用。PCT单抗效价达107，PCT蛋白与量子点偶联，具有较好稳定性。试纸条线性检测范围0.15–120 μg/L，灵敏度0.007 μg/L，回收率范围91%–113%，批内批间变异系数小于8%。自制的荧光检测试纸条与市场上已有的降钙素原ELISA试剂盒进行比对，检测结果良好，基本能够完成临床样品的检测。

Abstract:

Procalcitonin (PCT) is the precursor of calcitonin related to the severity of human bacterial infection. We made a test strip by coupling anti-PCT to quantum dot, in order to develop a highly sensitive and convenient PCT testing product. The anti-PCT titer had reached 107 because of the stability by coupling anti-PCT with quantum dot. The detecting linear range of the experiment was 0.15 to 120 μg/L, the sensitivity was 0.007 μg/L, the recovery range was 91% to 113%, and the intra- and inter-assay coefficient of variation was less than 8%. Comparing the homemade fluorescence-detected test strip with PCT ELISA kit on sale, we got accurate results which could mostly accomplish the test of clinical samples.