基于谱图库的蛋白质鉴定策略研究进展

Progress in the spectral library based protein identification strategy

摘要:

基于质谱的蛋白质组学快速发展，蛋白质质谱数据也呈指数式增长。寻找速度快、准确度高以及重复性好的鉴定方法是该领域的一项重要任务。谱图库搜索策略直接比较实验谱图与谱图库中的真实谱图，充分利用了谱图中的丰度、非常规碎裂模式和其他的一些特征，使得搜索更加快速和准确，成为蛋白质组学的主流鉴定方法之一。本文介绍基于谱图库的蛋白质组质谱数据鉴定策略，并针对其中两个关键步骤——谱图库构建方法和谱图库搜索方法进行深入介绍，探讨了谱图库策略的进展和挑战。

关键词:蛋白质鉴定，串联质谱，谱图库，谱图库搜索，谱图聚类

Abstract:

Exponential growth of the mass spectrometry (MS) data is exhibited when the mass spectrometry-based proteomics has been developing rapidly. It is a great challenge to develop some quick, accurate and repeatable methods to identify peptides and proteins. Nowadays, the spectral library searching has become a mature strategy for tandem mass spectra based proteins identification in proteomics, which searches the experiment spectra against a collection of confidently identified MS/MS spectra that have been observed previously, and fully utilizes the abundance in the spectrum, peaks from non-canonical fragment ions, and other features. This review provides an overview of the implement of spectral library search strategy, and two key steps, spectral library construction and spectral library searching comprehensively, and discusses the progress and challenge of the library search strategy.

Key words:protein identification, tandem mass spectrometry, spectral libraries, spectral library searching, spectrum clustering