大鼠、豚鼠和金黄地鼠的尿液蛋白质组比较

Comparison of urinary proteomes among rats, guinea pigs and golden hamsters

摘要:

生物标志物是指与生理或病理变化相关的可监测的变化。尿液作为机体的一种排泄物，不受稳态机制的调节，可以反映机体的多种变化。动物模型可以模拟人类疾病过程，监测疾病的变化，并为早期诊断提供线索。大鼠作为常用的模型动物并非所有疾病的优势模型动物，因此比较大鼠与其他动物的尿液蛋白质组，从而为其他疾病选择优势模型动物提供线索。文中通过膜上酶切切成肽段再通过液相色谱与串联质谱偶联技术 (LC-MS/MS)分析肽段信息，比较大鼠、豚鼠和金黄地鼠的尿液蛋白，结果显示3种鼠的尿蛋白数量不同，在机体不同系统中表达情况不同，参与的生物功能也不同。这为不同疾病选择不同的优势模型动物提供了依据。

Abstract:

Biomarkers are the detectable changes associated with physiological or pathological changes. Urine as excreta of the body, without the mechanisms to maintain a homeostatic internal environment, can reflect a variety of changes in the body. Using animal models can simulate human disease processes, monitor disease changes, and provide clues to early diagnosis. Rats as commonly used model animals are not the dominant models for all disease, thus comparing the urinary proteins of rats with other animals to provide clues to the selection of other animal dominant models. In this study, urinary proteins were digested and profiled by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The urinary proteins of rats, guinea pigs and golden hamsters were compared. The results showed that the number of urine proteins in the three different animals was different, and also different in every system of the body. This provides a basis for selecting the best animal models for different diseases.