基于甘油的1,3-丙二醇生物合成的代谢局限及其改造策略

Bottlenecks and modification strategies of 1,3-propanediol biosynthesis from glycerol

摘要:

粗甘油是生物柴油生产中的主要副产物，一些微生物可将甘油转化为重要化工原料1,3-丙二醇 (1,3-PD)，而利用这些微生物野生菌株生物合成1,3-PD会存在一些局限性，如底物抑制、产物抑制等。文中从1,3-丙二醇的甘油生物转化途径与这些局限性出发，总结了生物合成中存在的问题，并针对这些问题提出了一些基于基因敲除或基因过表达等基因工程技术的改造方法，综述了利用基因工程菌生物转化甘油生成1,3-丙二醇的最新研究进展。

Abstract:

Crude glycerol is the main by-product of biodiesel production. A few microorganisms can transfer crude glycerol to 1,3-propanediol (1,3-PD) that is an important chemical material. There exist many limitations such as substrate inhibition, product inhibition when wild strains are used in 1,3-PD biosynthesis. In this review, based on the microbial transformation of 1,3-propanediol from glycerol and its limitations, some strategies using genetic engineering such as knockout or gene overexpression were summarized. The latest research progresses in biosynthesis of 1,3-propanediol from glycerol by genetically engineered strains are discussed.