PKH26标记的人羊膜间充质干细胞在宫腔粘连大鼠中的迁移示踪

In vivo tracing of human amniotic mesenchymal stem cells labeled with PKH26 in rat intrauterine adhesions model

摘要:

为了观察PKH26标记的人羊膜间充质干细胞 (hAMSCs) 在宫腔粘连大鼠子宫内膜中的迁移情况，文中提取鉴定及PKH26标记hAMSCs，检测PKH26染色剂对hAMSCs生物学特性的影响；利用机械感染双重法建立大鼠宫腔粘连模型并经尾静脉移植PKH26标记的hAMSCs，荧光共聚焦显微镜下观察PKH26标记的hAMSCs移植后在大鼠子宫内膜中的分布情况。结果显示，PKH26染色剂对细胞的活性、周期、凋亡等无明显影响，PKH26标记的阳性细胞主要分布在大鼠受损的子宫内膜中。表明PKH26标记技术是一种安全有效的示踪方法，可用于hAMSCs移植在治疗宫腔粘连时的示踪研究。

Abstract:

To observe the migration of human amniotic mesenchymal stem cells (hAMSCs) labeled with PKH26 in the endometrium of rats intrauterine adhesion. hAMSCs were isolated, identified and labeled with PKH26 to detect the biological characteristics of the cells. Rat intrauterine adhesion models were established using mechanical and infective method and PKH26-labeled hAMSCs were transplanted through the tail vein. The distribution of PKH26 labeled hAMSCs in the endometrium of rats were observed with the fluorescence confocal microscope. The results showed that PKH26 stain had no significant effect on cell activity, cycle, apoptosis and so on. PKH26-labeled positive cells were mainly distributed in injured endometrium of rats. It shows that the PKH26 labeling technique is a safe and effective method for tracing the human amniotic mesenchymal stem cells in the treatment of intrauterine adhesions.