蒺藜苜蓿离体再生过程中MtSERK1的组蛋白修饰状态分析

Analysis of histone modification of MtSERK1 during in vitro regeneration in Medicago truncatula

摘要:

表观遗传修饰尤其是组蛋白修饰在维持植物基因组稳定、调控基因表达、促进离体再生等方面发挥了重要作用。MtSERK1基因是蒺藜苜蓿离体再生过程中胚性愈伤组织建立的重要标记基因。为了解该过程中组蛋白修饰与MtSERK1表达的动态调控关系，通过实时定量PCR方法分析了MtSERK1的表达变化并利用染色质免疫沉淀 (ChIP) 技术分析了其启动子区和不同基因结构区H3K9me2、H3K4me3和H3K9ac修饰状态。发现 MtSERK1在蒺藜苜蓿离体再生过程中的表达激活与其5′末端和3′末端区的组蛋白H3K4me3和H3K9ac修饰的动态变化相关。该研究将为深入了解MtSERK1参与蒺藜苜蓿离体再生的表达调控机制及其高效遗传转化体系的建立提供重要的理论指导。

Abstract:

Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation relationship between dynamic histone modification and MtSERK1s expression during the processes of in vitro organogenesis, the expression of MtSERK1 was analyzed by qRT-PCR, and the modification status of H3K9me2, H3K4me3 and H3K9ac in the promoter region and different regions included in the gene body was analyzed by chromatin immunoprecipitation (ChIP). We found expression activation of MtSERK1 was related to the dynamic changes of histone H3K4me3 and H3K9ac in the 5′ and 3′ regions. This study will provide important theoretical guidance for understanding of the regulatory mechanism of MtSERK1 and also for establishing efficient genetic transformation system of Medicago truncatula.