基于异肽键连接的分子粘合剂研究进展

Advances in isopeptide bond-mediated molecular superglue

摘要:

异肽键分子粘合剂是多肽链中的赖氨酸 (Lys) 残基和天冬酰胺或天冬氨酸 (Asn/Asp) 残基侧链自发结合形成的不可逆共价键，具有良好的特异性和稳定性。该反应可在极短的时间完成，且不需要特定的理化环境和催化剂。文中结合近年来国内外学者对异肽键分子粘合剂的研究进展，综述了异肽键分子粘合剂的来源、类型、形成机制及其介导的分子环化和蛋白拓扑结构，并讨论了其在合成疫苗、水凝胶和细菌纳米生物反应器等领域的应用前景。

Abstract:

Isopeptide bond-mediated molecular superglue is the irreversible covalent bond spontaneously formed by the side chains of lysine (Lys) and asparagine/aspartic acid (Asn/Asp) residues. The peptide-peptide interaction is specific, stable, and can be achieved quickly without any particular physicochemical factor. In the light of recent progress by domestic and foreign researchers, here we summarize the origin, assembly system and mechanism of isopeptide bond reaction, as well as the molecular cyclization and protein topological structure mediated by it. The prospect for its application in synthetic vaccine, hydrogel and bacterial nanobiological reactor is further discussed.