胰蛋白酶镜像酶LysargiNase的开发及其在蛋白质组学研究中的应用

Development of LysargiNase, a mirror trypsin and its application in proteomics

摘要:

蛋白质组学是系统鉴定、定量蛋白质及其翻译后修饰形式，并研究这些蛋白质生物学功能的学科。目前，基于质谱的鸟枪法蛋白质组学技术是蛋白质组学研究的主要手段之一，其技术流程是先将蛋白质组样品经位点特异性蛋白酶消化形成肽组，再进行高效液相色谱分离和质谱检测。而位点特异性蛋白酶对蛋白质样品的消化是质谱检测的前提和基础。随着蛋白质组学研究的深入，多种位点特异性蛋白酶被先后开发利用;而切割发生在相应氨基酸的N端，与传统的C端蛋白酶互为镜像的蛋白酶的鉴定、开发、特性研究和广泛使用更是为蛋白质组学研究提供了新的工具。文中对最近发现的胰蛋白酶的镜像酶——赖氨酸精氨酸N端蛋白酶（LysargiNase）的特点及其应用进行综述，为国内外学者更加广泛的使用创造条件。

Abstract:

Proteomics is a fast-growing discipline that aims at systematic identification, quantification of proteins and their post-translational modifications in cells. Mass spectrometry-based shotgun proteomics technology is currently one of the mainstream methods for proteomics research. With this method, proteins need to be digested to peptides by site-specific proteases before they can be detected with mass spectrometry. Therefore, site-specific proteases played key roles in this process and so far, a variety of specific proteases have been developed and used in proteomics study. Particularly, the identification, characterization and development of proteases that cleave at the N-termini of corresponding amino acid residues, which are just mirrors to those of typical C-termini proteases, provide novel tools for proteomics analysis. In this review, we summarized the proprieties of LysargiNase, a most recently identified mirror trypsin, and its applications in proteomics research to promote its more widespread usage.