手性自组装短肽对子宫创伤修复的影响

Chirality Self-assembling peptide for rats endometrial regeneration model

摘要:

本研究旨在探索手性自组装短肽在大鼠子宫创伤修复过程中发挥的作用。通过圆二色谱仪分析手性自组装短肽的二级结构；刚果红染色观察短肽自组装过程；红细胞裂解实验检测短肽对细胞膜的裂解作用；通过在模拟子宫创伤大鼠模型上引入手性自组装短肽，利用HE染色及免疫组织手段分析其在子宫创伤修复过程中的影响。结果显示，手性自组装短肽二级结构均为稳定的β折叠；可在盐离子触发下自组装形成致密的膜状结构；短肽自组装前后对细胞膜无裂解作用；可为细胞提供三维培养支架；Hela细胞在短肽形成的水凝胶环境中可持续生长；动物实验结果表明，手性自组装短肽可明显加快子宫修复过程。手性自组装短肽作为新型生物工程材料，可构建细胞三维培养环境并用于子宫创伤修复。

Abstract:

Here we investigate the physical and chemical properties of chiral self-assembling peptides and the role of uterine trauma regeneration. The circular dichroism was used to analyze secondary structure of chiral self-assembled peptide, and Congo red staining was used to observe the macroscopic process of peptide self-assembling. Erythrocyte lysis assay was used to examine the cleavage of peptide on cell membrane. The nanofiber scaffolds self-assembled by Chiral self-assembling peptides were used as the three-dimensional culture material to observe the growth effect of Hela cell. CCK-8 (cell counting kit-8) was used to study cell viability level between 2D (2-dimensional) and 3D (3-dimensional) culture environment. Rats endometrium curettage model was founded to evaluate the changes by immunohistochemistry staining and and HE staining. The secondary structure of chiral self-assembling peptides was stable β-sheet, and peptide could form dense membrane structure after 24 hours self-assembling cultured in salt ions. There was no harmful for the cell membrane of the peptide before and after self-assembling. Animal experiments show that chiral self-assembling peptide can significantly reduce the inflammatory response, promote the production of neovascularization, and accelerate the repair process. Chiral self-assembling peptide, as a new type of scaffold material, can construct a three-dimensional cell culture environment and used to repair uterine trauma.