微生物微培养系统研究现状与展望

Micro-cultivation system in microbiology: frontiers and prospects

摘要:

微生物培养是微生物学的起源和根本，成功的微生物培养是进行后续研究的重要前提与基础。但是，微生物培养操作不仅耗费了科研人员大量的精力和时间，还存在着极大的人为误差。近年来，容量小、高通量、模块化、可操作性良好并可进行在线检测的微生物微培养系统得到了微生物学领域的广泛关注。文中介绍了应用于微生物学领域的微培养系统，并按系统构成分类讨论了微生物微培养系统的发展、应用和展望。

关键词:微培养系统，微生物培养，自动化培养系统，模块化培养系统，微流控系统

Abstract:

Microbial cells cultivation is not only the origin, but also the foundation of microbiology. Researches in microbiology can only be carried out when the microbial cells can be cultured. However, conventional microbial cell cultivation is not only time consuming and labour intensive, but human error is also inevitable. Recent years, automated, modularised microbial cells micro-cultivation systems with small volume, good controllability, and equipped with real-time monitoring system have attracted great attention in microbiology. This review presents the state-of-the-art micro-cultivation systems which are implemented in microbial cells cultivation. The key development, applications of various system classified based on their construction, and the prospects of micro-cultivation system are discussed and insights into them are also provided.

Key words:micro-cultivation system, microbial cells cultivation, automated cultivation system, modularised cultivation system, microfluidic systems