酶法转化生产α-酮酸的研究进展

Recent advances in enzymatic production of alpha-keto acids

摘要:

α-酮酸是一种同时含有羧基和酮基的双官能团有机化合物，广泛应用于食品、药品和化妆品等行业。为了满足环境友好、安全高效和可持续发展的社会要求，利用酶转化法生产α-酮酸受到人们的广泛关注。文中从酶的筛选、酶的改造以及酶的转化条件优化3个方面介绍丙酮酸、α-酮戊二酸、酮亮氨酸、酮缬氨酸、苯丙酮酸和酮蛋氨酸酶法合成的研究状况，并展望了α-酮酸进一步高效生产的发展方向。

Abstract:

Alpha-keto acid is a bifunctional organic compound containing both carboxyl and ketone groups, and widely applied in the industries of food, pharmaceutical and cosmetics. Based on the demand of eco-friendly process, safety and sustainable development, production of α-keto acids by enzymatic conversion technology has been paid more and more attention. In this article, we review the status of α-keto acids biosynthesis from three aspects: enzymatic screening, enzymatic modification and optimization of enzymatic conversion conditions. Meanwhile, we also indicate future research directions for further improving α-keto acids production．