3¢-磷酸腺苷-5¢-磷酸硫酸的高效合成及其应用

Production and application of 3¢-phosphoadenosine-5¢-phosphosulfate

摘要:

硫酸化化合物广泛存在于胞浆、细胞表面及胞外基质中，在机体细胞发育、分化、免疫、解毒和信号传递等生命活动过程中起着不可替代的作用。3￠-磷酸腺苷-5￠-磷酸硫酸 (3￠-phosphoadenosine-5￠-phosphosulfate，PAPS)是化合物硫酸化过程中最常用的硫酸基供体，但目前合成PAPS并最终实现其工业化应用还困难重重。文中主要综述过去10年内关于PAPS的生物合成及应用的研究进展，以期为PAPS的合成及其在芥子油苷、肝素、硫酸软骨素及羟胺硝喹等的生物合成中的应用提供参考。

Abstract:

Sulfated compounds are widely present in cytoplasm, on cell surface, and in extracellular matrix. These compounds play important roles in cell development, differentiation, immune response, detoxication, and cell signal transduction. 3￠-Phosphoadenosine-5￠-phosphosulfate (PAPS) is the universal sulfate group donor for the biosynthesis of sulfated compounds. Up to now, the synthesis of PAPS is still too expensive for industrial applications. This review focuses on the recent progress of PAPS production and summaries the application of PAPS, particularly in the production of glucosinolate, heparin, condroitin sulfate, and oxamniquine production.