微生物1,3-1,4-β-葡聚糖酶蛋白质改造及工业应用研究进展

Research progresses in microbial 1,3-1,4-β-glucanase: protein engineering and industrial applications

摘要:

1,3-1,4-β-葡聚糖酶 (E.C.3.2.1.73) 是一种重要的工业用酶，其可以通过特异性切割毗邻β-1,3-糖苷键的β-1,4-糖苷键将β-葡聚糖或地衣多糖降解为纤维三糖和纤维四糖。微生物β-葡聚糖酶属于糖苷水解酶家族16，其三维结构为卷心蛋糕状的逆向β-片层结构。文中综述了近些年来β-葡聚糖酶在工业上的应用情况及酶蛋白质工程改造的研究进展，并对其研究前景进行了展望。

关键词:1,3-1,4-β-葡聚糖酶，工业应用，蛋白质改造，稳定性，催化性质

Abstract:

1,3-1,4-β-glucanase (E.C.3.2.1.73) is an important industrial enzyme which cleave β-glucans into oligosaccharides through strictly cutting the β-1,4 glycosidic bonds in 3-O-substituted glucopyranose units. Microbial 1,3-1,4-β-glucanase belongs to retaining glycosyl hydrolases of family 16 with a jellyroll β-sandwich fold structure. The present paper reviews the industrial application and protein engineering of microbial β-glucanases in the last decades and forecasts the research prospects of microbial β-glucanases.

Key words:1,3-1,4-β-glucanase, industrial application, protein engineering, stability, catalytic property