磷铵两性离子改性血管脱细胞支架可以有效体外抗血栓和内皮化

Acellular vascular scaffolds modified by ammonium phosphate zwitterions can effectively resist thrombosis and promote endothelialization in vitro

摘要:

由于自体血管 (由同一受体的血管用于血管移植材料) 的有限可用性，以及非自体血管 (人工制成的血管移植材料) 的生长能力不足，组织工程血管越来越受到重视。文中构建了一种磷铵两性离子改性的血管脱细胞支架附以高度生物相容的骨髓源内皮祖细胞为内层的新型血管移植材料。通过一种简便的方法——共沉淀法改性血管脱细胞支架，评价其体外血小板粘附实验、溶血实验、复钙实验和细胞毒性等相关指标。磷铵两性离子改性后抗凝血活性提高，可以有效地促使类似于天然血管腔表面凹凸结构的脱细胞支架表面内皮祖细胞的附着。改性后的脱细胞支架具有与天然血管相似的力学性能，在体外可以有效地构建内皮化。研究结果为血管脱细胞支架通过改性实现体外抗血栓和内皮化方面进行了初步探索。

Abstract:

Due to limited availability of autologous blood vessels (blood vessels from the same recipient used for vascular transplantation materials) and inadequate growth ability of non-autologous blood vessels (artificial blood vessel transplantation materials), more and more attention has been paid to tissue engineering blood vessels. In this study, we constructed an ammonium phosphate zwitterion modified acellular vascular scaffold with highly biocompatible bone marrow-derived endothelial progenitor cells as the inner layer of a new vascular transplantation material. The vascular acellular scaffolds were modified by a simple method—co-precipitation method. The platelet adhesion test, hemolysis test, recalcification test and cytotoxicity of acellular vascular scaffolds in vitro were evaluated. Ammonium phosphate zwitterions modified endothelial progenitor cells on the surface of acellular scaffolds with concave and convex structure on the surface of natural vascular lumen can be effectively promoted by improving anticoagulant activity. Modified acellular scaffolds have similar mechanical properties to natural blood vessels and can effectively construct endothelialization in vitro. The results of this study provide a preliminary exploration for the modification of vascular acellular scaffolds to achieve anti-thrombosis and endothelialization in vitro.