HIV-1 Env三聚体抗原改造研究进展

Progress in HIV-1 Env trimer design

摘要:

HIV-1疫苗研发是当前艾滋病研究的一大热点，其病毒表面包膜糖蛋白Env三聚体介导病毒与细胞融合，是HIV-1疫苗研究的重要靶点。因此，设计并在体外表达类天然Env三聚体对HIV-1疫苗的研发具有重要的意义。近年来，Env三聚体研究取得了显著的进展。SOSIP、NFL2P、UFO等抗原改造方法实现了类天然Env三聚体的体外表达，逐步解决了改造抗原产量低、结构不稳定等问题，且表达的Env三聚体抗原能在动物免疫中诱导机体产生较高的中和抗体水平。Env三聚体改造方法促进了HIV-1疫苗的研究。文中综述了SOSIP、NFL2P、UFO三种HIV-1 Env三聚体抗原改造方法，对比各个改造方法优缺点，并结合自身工作提出相关建议，为后续HIV-1抗原的相关设计提供指导。

关键词:人类免疫缺陷病毒I型，Env三聚体，抗原设计，中和抗体

Abstract:

Currently, HIV-1 vaccine development has still been a hot pot in the AIDS research. HIV-1 glycoprotein Env is the sole target in the virion surface that mediates the membrane fusion between the virion and cell in the HIV-1 infection process. Env protein is the significant immunogen for HIV-1 vaccine development. In recent years, there have been breakthroughs in the Env trimer research. For example, the strategies including SOSIP, NFL2P, and UFO had been applied to design and generate HIV-1 Env trimer. The improvement of quantity and stability is beneficial to achieve the HIV-1 native-like Env trimer for elicitation of strong neutralizing antibody responsing in animal immunization. This review focuses on the different strategies for Env trimer design and compares their advantages and disadvantages, combining with our work to give some advice, which might provide relevant information for the future HIV-1 immunogen design.

Key words:HIV-1, Env trimer, antigen design, neutralizing antibody