一种可止血的富血小板血浆壳聚糖/丝素蛋白多孔伤口敷料的制备及性能表征

Preparation and characterization of a hemostatic porous platelet-rich plasma chitosan/silk fibroin wound dressing

摘要:

为了进一步提高伤口敷料的止血性能，文中在生物相容性良好的壳聚糖溶液中引入含有多种生长因子的人源性富血小板血浆 (Human platelet-rich plasma，hPRP)，并加入不同体积比例 (1∶1、1∶3、3∶1、1∶0) 的丝素蛋白溶液以提高材料的多孔性与止血性，通过冷冻干燥法制备不同配比的hPRP-壳聚糖/丝素蛋白敷料，并将纯壳聚糖敷料作为对照组，研究hPRP和丝素蛋白对敷料的止血性能的影响以及丝素蛋白对PRP中生长因子控制释放的影响。结果表明，在壳聚糖敷料中引入hPRP对敷料的止血性有所提高，但对敷料的多孔结构及吸水率无明显改善，若在hPRP-壳聚糖溶液中按照体积比为1∶1的比例加入丝素蛋白溶液，会得到具有较为均匀的多孔结构的敷料，敷料的孔隙率与吸水率分别可达到86.83%±3.84%与1 474%±114%，且该比例的敷料在快速止血性能上表现优异。此外，加入丝素蛋白与壳聚糖比例为1∶1的PRP敷料能有效减少PRP中生长因子在初始阶段的爆裂释放。因此，含hPRP的壳聚糖/丝素蛋白复合敷料有望成为一种能快速止血且能促进伤口愈合的新型伤口敷料。

Abstract:

In order to further improve the hemostatic performance of wound dressings, human platelet-rich plasma (PRP) containing various growth factors was introduced into chitosan solution. The silk fibroin solutions with different volume ratios (1:1, 1:3, 3:1 and 1:0) were added to improve the porosity and hemostasis of materials. The hPRP-chitosan/silk wound dressings with different ratios was prepared by freeze-drying and pure chitosan dressing was considered as the control group to study the effects of PRP and silk fibroin in different proportions on the hemostasis properties and the growth factors burst release. The hemostasis of chitosan dressing was improved by introducing PRP, but the porous structure and water absorption were not significantly improved. If silk fibroin solution was added in the ratio of 1:1, the more uniform porous structure and better hemostatic performance could be obtained. The porosity and water absorption could reach 86.83%±3.84% and 1 474%±114% respectively. In addition, the dressings with ratio of 1:1 had positive effects on reducing the burst release of growth factors on initial stage. Therefore, PRP-chitosan/silk fibroin composite dressing can become a kind of wound dressing that can achieve rapid hemostasis and promote wound healing.