卵泡液中细胞外囊泡及其携带的microRNA对卵泡发育的作用

Effect of extracellular vesicles and microRNAs in follicular fluid on follicular development

摘要:

细胞外囊泡 (Extracellular vesicles，EVs) 是指细胞分泌的双层膜转运囊泡。EVs能从细胞中摄取大分子物质，并将其转移至受体细胞。在这些大分子物质中，研究最多的就是microRNA (miRNA)。miRNA是一种参与基因表达调控的非编码RNA，已证实在哺乳动物卵泡液EVs中有不同的非编码RNA存在，EVs携带miRNA可以作为自分泌和旁分泌的替代机制，影响卵泡发育。文中系统介绍了EVs的种类、特征和分离鉴定方法，重点综述了EVs及携带的miRNA对卵泡发育的作用，包括早期卵泡发育、卵母细胞成熟、卵泡优势化以及对颗粒细胞功能的影响。同时对卵泡液中EVs及其携带的miRNA的未来研究进行了展望，为卵泡液中EVs及携带的miRNA功能的研究及应用提供了思路和方向。

Abstract:

Extracellular vesicles (EVs) refer to bilayer membrane transport vesicles secreted by cells. EVs can take macromolecules from cells and transfer them to receptor cells. Among these macromolecular substances, the most studied are microRNAs (miRNAs). miRNA is non-coding RNA involved in the regulation of gene expression. It has been confirmed that there are different non-coding RNAs in mammalian follicular fluid EVs. EVs carrying miRNA can act as an alternative mechanism for autocrine and paracrine, affecting follicular development. This paper systematically introduced the kinds, characteristics and methods of isolation and identification of EVs, focusing on the effects of EVs and miRNAs on follicular development, including early follicular development, oocyte maturation, follicular dominance and effects on granulosa cell function. At the same time, the authors prospected the future research of EVs and microRNAs in follicular fluid, and provided ideas and directions for the research and application of EVs and miRNA functions in follicular fluid.