SCD-1基因过表达对鸭子宫上皮细胞钙离子和脂质含量的效应

Effects of SCD-1 gene overexpression on the content of calcium ion and lipids in duck uterine epithelial cells

摘要:

硬脂酰辅酶A去饱和酶-1 (Stearoyl-CoA desaturase-1，SCD-1) 是催化单不饱和脂肪酸合成的关键性蛋白酶，Ca2+是生物体内重要阳离子，在生物体内发挥着重要作用。为探讨SCD-1基因与脂质指标和钙离子含量之间的关联性，通过构建pcDNA3.1(+)+SCD-1+Flag真核过表达载体和培养鸭子宫上皮细胞并共转染，通过载体上Flag标签检测SCD-1基因的过表达量，用Fluo-3/AM钙离子荧光标记法检测Ca2+浓度，用脂质指标试剂盒检测细胞内的脂质含量。结果表明，鸭SCD-1基因过表达量与甘油三酯 (TG) 和高密度脂蛋白胆固醇 (HDL-C) 含量呈负相关，与Ca2+浓度、总胆固醇 (TC) 含量、极低密度脂蛋白胆固醇 (VLDL-C) 含量和低密度脂蛋白胆固醇 (LDL-C) 含量呈正相关；Ca2+与TG、LDL-C和HDL-C的含量呈正相关，与TC和VLDL-C含量呈负相关，研究结果揭示了SCD-1基因过表达能够调控鸭子宫上皮细胞中Ca2＋浓度和脂质合成与转运。

Abstract:

Stearoyl-CoAdesaturase-1 (SCD-1) is a key regulator of monounsaturated fatty acid synthesis. It plays a vital role in lipid synthesis and metabolism. Ca2+ is an important cation in the body and plays an important role in the organism. The aims of this study were to investigate the correlation of SCD-1 gene overexpression with lipid indexes and calcium ion level. The pcDNA3.1 (+) + SCD-1 +Flag eukaryotic expression vector and cultured duck uterine epithelial cells were co-transfected. The overexpression of SCD-1 gene was measured using the Flag Label Detection Kit. Ca ions and lipid contents were detected through Fluo-3/AM Calcium Ion Fluorescence Labeling method and Lipid Measuring Kit, respectively. SCD-1 gene overexpression was negatively correlated with triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C), and positively correlated with Ca ion, total cholesterol (TC), very low-density lipoprotein cholesterol (VLDL-C) and low density lipoprotein cholesterol (LDL-C) levels. Meanwhile, Ca ion was positively correlated with TG, LDL-C and HDL-C contents, and negatively correlated with TC and VLDL-C levels. Overexpression of SCD-1 gene could regulate Ca ion secretion, as well as lipid synthesis and transport in duck uterine epithelial cells.