苜蓿链霉菌内切β-N-乙酰氨基葡萄糖苷酶的克隆、表达及酶学性质

Cloning, expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae

摘要:

内切β-N-乙酰氨基葡萄糖苷酶广泛应用于糖生物学研究和工业生产。本研究从苜蓿链霉菌Streptomyces alfalfae ACCC 40021中克隆并原核表达了一个新的内切β-N-乙酰氨基葡萄糖苷酶，该酶最适反应温度为35 ℃，最适pH为6.0，具有良好的pH稳定性、温度稳定性和高比活（1×106 U/mg）的特性，可催化不同蛋白底物去糖基化，具有作为工具酶和生物催化剂的潜力。

Abstract:

Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries. In this study, a new endo-β-N-acetylglucosaminidase, designated as Endo SA, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21 (DE3). The purified recombinant Endo SA exhibited the maximum activity at 35 oC and pH 6.0, good thermo/pH stability and high specific activity (1.0×106 U/mg). It displayed deglycosylation activity towards different protein substrates. These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.