生物催化甲醛生成L-木糖

Biocatalysis of formaldehyde to L-xylose

摘要:

在当今不可再生资源日益消耗的情形下，利用生物合成的技术，将甲醛转变成糖类，具有重要意义。该过程最重要的是找到一个合适的催化剂组合来实现甲醛的二聚反应。在最近的研究中，报道发现了一种乙醇醛合酶 (Glycolaldehyde synthase，GALS) 可以催化这一反应，将其与D-果糖-6磷酸醛缩酶 (D-fructose-6-phosphate aldolase，FSA) 组合使用，即“一锅酶”法，可以利用甲醛合成L-木糖，并且转化率可达64%。这一过程的实现也为合成其他糖的反应提供了参考。

Abstract:

It is of great significance to use biosynthesis to transform the inorganic substance formaldehyde into organic sugars. Most important in this process was to find a suitable catalyst combination to achieve the dimerization of formaldehyde. In a recent report, an engineered glycolaldehyde synthase was reported to catalyze this reaction. It could be combined with engineered D-fructose-6-phosphate aldolase, a “one-pot enzyme” method, to synthesize L-xylose using formaldehyde and the conversion rate could reach up to 64%. This process also provides a reference for the synthesis of other sugars. With the increasing consumption of non-renewable resources, it was of great significance to convert formaldehyde into sugar by biosynthesis.