哺乳动物细胞灌流培养工艺开发与优化

Development and optimization of perfusion process for mammalian cell culture

摘要:

近年来生物药市场需求量激增，高产量、高质量、低成本的哺乳动物细胞灌流培养工艺顺势成为工业界和学术界普遍关注的热点。文中围绕灌流培养工艺特有的操作环节及工艺优化应着重关注的细节展开论述，综述了近年来在灌流培养工艺开发和优化上取得的进步和提出的策略，以期为哺乳动物细胞灌流培养技术的开发提供参考。

Abstract:

In recent years, the demand of biologics has increased rapidly. Cell culture process with perfusion mode has become more and more popular due to its high productivity, good quality and high efficiency. In this paper, the unique operation and the details of process optimization for perfusion culture mode are discussed by comparing with traditional batch culture process. Meanwhile, the progress and strategies in the development and optimization of perfusion culture process in recent years are summarized to provide reference for the future development of mammalian cell perfusion culture technology.