生物合成3-羟基丙酸的代谢工程研究进展

Progress in metabolic engineering of biosynthesis of 3-hydroxypropionic acid

摘要:

3-羟基丙酸 (3-HP) 作为一种重要的平台化合物，以此为底物能够合成多种具有商业潜质的生物制品。野生菌合成3-HP产量较低，严重限制3-HP的大规模应用与生产，通过改造合成代谢通路的相关基因，构建以廉价底物为碳源的工程菌株，实现降低生产成本提高产量的目的。文中将对近年来国内外通过代谢工程合成3-羟基丙酸的研究进展进行概述，并对甘油途径、丙二酸单酰辅酶A途径、β-丙氨酸等途径合成3-HP的优缺点进行总结分析，对3-HP未来发展前景进行展望。

Abstract:

As an important platform compound, 3-hydroxypropionic acid (3-HP) can be used as a substrate to synthesize a variety of biological products with commercial potential. The titer of 3-HP by wild-type bacteria is low, which severely limits the large-scale application and production of 3-HP. By modifying the genes related to the metabolic pathway, engineered bacteria using cheap substrates as carbon sources are constructed, the aim of reducing production cost and increasing output is realized. In this paper, the recent progress in the synthesis of 3-HP by metabolic engineering at home and abroad is reviewed. The advantages and disadvantages of glycerol pathway, malonyl-CoA pathway and beta-alanine pathway for synthesis of 3-HP are also summarized and analyzed, and the future development of 3-HP is prospected.