乳酸乳球菌NZ9000基因组规模代谢网络模型的构建与验证

Construction and verification of Lactococcus lactis NZ9000 genome-scale metabolic model

摘要:

随着后基因组时代的到来，工业微生物的代谢工程改造在工业生产上发挥着越来越重要的作用。而基因组规模代谢网络模型 (Genome-scale metabolic model，GSMM) 将生物体体内所有已知代谢信息进行整合，为全局理解生物体的代谢状态、理性指导代谢工程改造提供了最佳的平台。乳酸乳球菌NZ9000 (Lactococcus lactis NZ9000) 作为工业发酵领域的重要菌株之一，由于其遗传背景清晰且几乎不分泌蛋白，是基因工程改造和外源蛋白表达的理想模式菌株。文中基于基因组功能注释和比较基因组学构建了L. lactis NZ9000的首个基因组规模代谢网络模型iWK557，包含557个基因、668个代谢物、840个反应，并进一步在定性和定量两个层次验证了iWK557的准确性，以期为理性指导L. lactis NZ9000代谢工程改造提供良好工具。

Abstract:

With the advent of the post-genomic era, metabolic engineering of microorganisms plays an increasingly important role in industrial production. The genome-scale metabolic model (GSMM) integrates all known metabolic information in the organism to provide an optimal platform for global understanding of the metabolic state of the organism and rational guidance for metabolic engineering. As a model strain, Lactococcus lactis NZ9000 plays an important role in industrial fermentation, but there is still no specific genome-scale metabolic model for it. Based on genomic function annotation and comparative genomics, we constructed the first genome-scale metabolic model iWK557 of L. lactis NZ9000, which contains 557 genes, 668 metabolites, and 840 reactions, and further verified at both qualitative and quantitative levels, to provide a good tool for rationally guiding metabolic engineering.