金色链霉菌DM-1全基因组序列测定及去甲金霉素合成基因簇分析

Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster

摘要:

金色链霉菌Streptomyces aureofaciens DM-1是去甲基金霉素的高产菌株。通过Genome Sequencer FLX系统进行测序，得到一条完整的线性基因组序列，长度为6 824 334 bp，GC含量为72.6%。结合软件glimmer 3.02、Genemark和Z-Curve program进行基因预测，最终在其基因组中鉴定出6 431个基因。应用AntiSMASH软件预测其基因组中存在28个次级代谢生物合成基因簇，其中包含了去甲基金霉素生物合成基因簇。其中甲基转移酶CtcK因移码突变提前终止翻译，很可能是去甲基金霉素相对金霉素 (CTC) 缺失一个甲基的根本原因。研究结果为S. aureofaciens DM-1的功能基因组学和去甲基金霉素高产菌株育种提供了研究基础。

Abstract:

Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer. The genome sequencing of DM-1 reveals a linear chromosome containing 6 824 334 bps nucleotides with GC content of 72.6%. In this genome, a total of 6 431 open reading frames were predicted by using glimmer 3.02, Genemark and Z-Curve softwares. Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software, including the complete 6-demethylchlortetracycline biosynthetic gene cluster. A frame-shift mutation in methyltransferase coding region was detected, which may result in the demethylation of chlortetracycline. The complete genome sequence of S. aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline.