海生杆菌属的基因组测序数据分析

Analysis of the genome sequencing data of the Marinobacterium genus

摘要:

海生杆菌属首次于1997年鉴定，迄今包括18个物种，其中10个已完成全基因组序列测定。文中总结了海生杆菌属的菌种特征，并从碳源利用、聚羟基脂肪酸酯代谢和芳香族化合物降解三个方面对基因组测序数据进行了分析。研究发现，海生杆菌属具有完整的糖酵解途径和三羧酸循环，缺乏木糖利用基因。所有海生杆菌属菌种均含有Ⅰ型和Ⅲ型聚羟基脂肪酸酯合成酶的编码基因，表明该菌属可能具有普遍的聚羟基脂肪酸酯合成能力。海生杆菌属含有芳香族化合物的降解途径，苯、苯酚和苯甲酸可由不同的酶催化生成邻苯二酚，再由邻位断裂途径降解为3-酮己二酸，邻苯二酚也可由间位断裂途径降解为丙酮酸和乙酰辅酶A。基因组测序数据分析加深了对海生杆菌属代谢特征的认识，提示该菌属在聚羟基脂肪酸酯合成和海洋芳香族污染物治理方面有一定的应用前景。

Abstract:

The marine genus Marinobacterium was first identified in 1997, and a total of 18 species have been characterized so far, 10 of which have published whole-genome sequencing data. This article summarizes the characteristics of Marinobacterium genus and analyzes the genome sequencing data related to the carbon source utilization, polyhydroxyalkanoate metabolism, and aromatic compounds degradation. The Marinobacterium species possess the complete glycolysis pathway and tricarboxylic acid cycle, yet lack genes involved in xylose utilization. All strains of the Marinobacterium genus contain the genes encoding for the typeⅠand type Ⅲ polyhydroxyalkanoate synthases, suggesting that the genus may have ability of polyhydroxyalkanoate accumulation. The Marinobacterium species contain the degradation pathways of aromatic compounds. Benzene, phenol and benzoic acid can be degraded into catechol via different enzymes, subsequently catechol is converted to 3-ketoadipate through the ortho-cleavage pathway. Alternatively, catechol can be degraded into pyruvate and acetyl-CoA. The analysis of genome sequencing data of the Marinobacterium genus provides in-depth understanding of the metabolic characteristics, indicating that the genus may have certain applications in the synthesis of polyhydroxyalkanoate and the removal of marine aromatic compounds.