酪氨酸磷酸化蛋白质组学技术研究进展及其在生物医学研究中的应用

Application of tyrosine phosphoproteomics in biomedical research: a review

摘要:

在酪氨酸磷酸化蛋白质组学的研究过程中，酪氨酸磷酸化位点的富集是最重要的一步。目前常用的富集方法是抗体亲和富集或SH2 superbinder富集。此外，通过质谱与生物信息学等技术，可实现大规模酪氨酸磷酸化位点的鉴定。对酪氨酸磷酸化蛋白质组学进行深度覆盖研究，揭示癌症发生发展过程中失调的激酶，将有助于深入理解癌症的发生发展过程；且由于75%的致癌基因是酪氨酸激酶基因，酪氨酸激酶抑制剂作为抗癌药物受到了越来越多的关注。应用酪氨酸磷酸化蛋白质组学技术，可以鉴定与癌症等重大疾病相关的酪氨酸激酶，从而帮助找到酪氨酸激酶抑制剂。总之，酪氨酸磷酸化蛋白质组学技术可以在酪氨酸激酶鉴定、酪氨酸激酶抑制剂研究及酪氨酸磷酸化信号通路研究等生物医学领域中得到很好的应用。

关键词:酪氨酸激酶，酪氨酸磷酸化，酪氨酸磷酸化肽段富集，精准医学

Abstract:

The enrichment of tyrosine phosphorylation sites plays an important role in the study of tyrosine phosphoproteomics and the commonly used enrichment methods are antibody affinity enrichment and SH2 superbinder enrichment. In addition, in order to achieve large-scale identification of tyrosine phosphorylation sites, biological mass spectrometry and bioinformatics have been applied in tyrosine phosphoproteomics. In-depth coverage research of tyrosine phosphoproteomics, revealing the dysregulated kinases in cancer process, may help us understanding the occurrence and development process of cancer. According to literature reports, three quarters of the oncogenes are tyrosine kinase genes. Therefore, tyrosine kinase inhibitors have received more and more attention as anticancer drugs. The application of tyrosine phosphoproteomics technology can identify tyrosine kinases related to cancer and other major diseases, so as to help finding tyrosine kinase inhibitors. In short, tyrosine phosphoproteomics technology can be applied in biomedical fields such as tyrosine kinase identification, tyrosine kinase inhibitor research, and tyrosine phosphorylation signal pathway research.

Key words:tyrosine kinase, tyrosine phosphorylation, enrichment of tyrosine phosphorylation peptide, precision medicine