蔗糖磷酸化酶及其在糖基化反应中的应用

Application of sucrose phosphorylase in glycosylation

摘要:

糖基化反应能有效改善化合物的水溶性、稳定性、生物利用度等性质，利用糖苷水解酶类和糖基转移酶类对生物活性化合物进行糖基化修饰已成为研究热点。相比于糖基转移酶类，糖苷水解酶类在大规模催化中具有来源丰富、成本低的优势。其中，蔗糖磷酸化酶因其卓越的糖基化活性和广泛的底物特异性，在化工领域受到人们的广泛关注。文中综述了蔗糖磷酸化酶的结构与催化特性，概述了蔗糖磷酸化酶的定向改造，同时系统性地总结了蔗糖磷酸化酶在糖基化反应中的应用及与其他酶的联合应用。并且，基于蔗糖磷酸化酶的研究现状，结合笔者研究团队的多年工作经验，探讨了该课题的未来发展方向。

Abstract:

Water solubility, stability, and bioavailability, can be substantially improved after glycosylation. Glycosylation of bioactive compounds catalyzed by glycoside hydrolases (GHs) and glycosyltransferases (GTs) has become a research hotspot. Thanks to their rich sources and use of cheap glycosyl donors, GHs are advantageous in terms of scaled catalysis compared to GTs. Among GHs, sucrose phosphorylase has attracted extensive attentions in chemical engineering due to its prominent glycosylation activity as well as its acceptor promiscuity. This paper reviews the structure, catalytic characteristics, and directional redesign of sucrose phosphorylase. Meanwhile, glycosylation of diverse chemicals with sucrose phosphorylase and its coupling applications with other biocatalysts are summarized. Future research directions were also discussed based on the current research progress combined with our working experience.