随机突变文库构建与筛选研究进展

Progress in the construction and screening of random mutation library

摘要:

定向进化是一个循环过程，在构建多样化基因序列和筛选功能基因变体之间交替进行。该技术目前已被广泛应用于DNA序列、基因功能和蛋白质结构的优化和分析。定向进化包括随机基因文库的生成、基因在合适宿主中的表达和突变文库的筛选。构建基因文库的关键是库容量和突变多样性，而筛选变体的关键是高灵敏度和高通量。文中讨论了定向进化技术的最新进展，这些新技术极大地加速和简化了传统的定向进化过程，为定向进化的发展注入了强劲动力。

Abstract:

Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants. It has been widely used in optimization and analysis of DNA sequence, gene function and protein structure. It includes random gene libraries construction, gene expression in suitable hosts and mutant libraries screening. The key to construct gene library is the storage capacity and mutation diversity, to screen is high sensitivity and high throughput. This review discusses the latest advances in directed evolution. These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.