小鼠生长过程中肝、肺、肾胶原蛋白与MMP-1含量变化

Changes of collagen and MMP-1 in liver, lung and kidney during growth of mice

摘要:

利用高效液相色谱法 (High performance liquid chromatography，HPLC) 和酶联免疫吸附实验 (Enzyme-linked immunoassay，ELISA) 对小鼠生长过程中肝、肺、肾胶原蛋白与基质金属蛋白酶-1 (Matrix metalloproteinase-1，MMP-1) 含量变化规律进行研究。以0–18周的小鼠肝、肺、肾为研究对象，通过HPLC法对不同周龄小鼠肝、肺、肾中羟脯胺酸 (Hydroxyproline，Hyp) 含量及比例进行测定，并换算得到胶原蛋白的含量；利用ELISA检测不同周龄小鼠肝、肺、肾中MMP-1的含量和活性。结果表明，小鼠肝、肺、肾中胶原含量各不相同(肺 (COL)>肾 (COL)>肝 (COL))，且随着周龄的增加胶原蛋白含量变化均呈先增加后降低的趋势。其中肝、肺、肾中胶原蛋白含量分别在第9、6、9周达到最高值，分别为5.52 ng/mg、54.10 ng/mg、19.20 ng/mg；9–18周呈缓慢降低趋势。ELISA检测结果表明，小鼠肝、肺、肾中MMP-1的含量随周龄增加呈先降低后增加的趋势，MMP-1活性的变化趋势与之相反。这表明组织内胶原蛋白含量的增加会抑制MMP-1的分泌。

Abstract:

The high performance liquid chromatography (HPLC) and enzyme-linked immunoassay (ELISA) were used to investigate the changes of collagen and matrix metalloproteinase-1 (MMP-1) in liver, lung and kidney during growth process of mice. The mice from 0 to 18 weeks were used as the research objects. The contents and proportions of hydroxyproline (Hyp), which were used to calculate the collagen contents, in liver, lung and kidney of different weeks were analyzed with HPLC. The contents and activity of MMP-1 in liver, lung and kidney of different weeks were analyzed with ELISA. The results showed that the collagen contents in liver, lung, and kidney were different (Lung(COL)>Kidney(COL)>Liver(COL)), and they all increased first and then decreased with weeks. The collagen contents in liver, lung, and kidney reached the highest level in the ninth (5.52 ng/mg), sixth (54.10 ng/mg) and ninth (19.20 ng/mg) week, respectively. Then it declined slowly from 9 to 18 weeks. The result of ELISA showed that the MMP-1 contents in liver, lung and kidney decreased first and then increased with weeks, and the trend of MMP-1 activity was opposite. It indicated that the increase of collagen contents in the tissues will inhibit the secretion of MMP-1.