青霉生产木质纤维素降解酶系的研究进展

Progress in the production of lignocellulolytic enzyme systems using Penicillium species

摘要:

木质纤维素降解酶系的高效生产是实现植物生物质大规模生物炼制的重要支撑。就地生产木质纤维素降解酶，有助于降低其使用成本，提高技术经济效益。青霉是自然界常见的木质纤维素降解真菌，可以合成分泌种类多样、组分齐全的木质纤维素降解酶系，已被应用于纤维素酶制剂的工业生产。文中从就地生产降解酶，为木质纤维素生物炼制构建“糖平台”的角度，综述了青霉木质纤维素降解酶系的性质、菌株遗传改造及发酵工艺的研究进展。

Abstract:

The efficient production of lignocellulolytic enzyme systems is an important support for large-scale biorefinery of plant biomass. On-site production of lignocellulolytic enzymes could increase the economic benefits of the process by lowering the cost of enzyme usage. Penicillium species are commonly found lignocellulose-degrading fungi in nature, and have been used for industrial production of cellulase preparations due to their abilities to secrete complete and well-balanced lignocellulolytic enzyme systems. Here, we introduce the reported Penicillium species for cellulase production, summarize the characteristics of their enzymes, and describe the strategies of strain engineering for improving the production and performance of lignocellulolytic enzymes. We also review the progress in fermentation process optimization regarding the on-site production of lignocellulolytic enzymes using Penicillium species, and suggest prospect of future work from the perspective of building a “sugar platform” for the biorefinery of lignocellulosic biomass.