无化学修饰的小分子药物靶蛋白鉴定技术及其应用进展

Identification techniques of small molecule drug target proteins without chemical modification and its applications: a review

摘要:

鉴定小分子药物的靶蛋白对于理解药物的作用机理以及药物副作用至关重要。传统方法需要对药物进行化学修饰共价交联，可能会导致药物活性的改变。目前已经发展多种无需化学修饰便可以对药物靶蛋白鉴定的方法，包括药物亲和力反应靶标稳定性技术 (Drug affinity responsive target stability，DARTS)、蛋白质氧化速率稳定性技术 (Stability of proteins from rates of oxidation，SPROX)、细胞热移位分析技术 (Cellular thermal shift assay，CETSA) 和热蛋白组分析技术 (Thermal proteome profiling，TPP) 等。文中将介绍这些技术的原理、应用以及各自的优点和局限性，另外也介绍了这些技术最新的优化方案。

Abstract:

Identification of the target proteins of small molecule drugs is crucial for understanding the mechanisms of drug actions and its side effects. Conventional methods require chemical modification, which might alter the activities of the drugs. Various label-free techniques have been developed to identify drug target proteins without chemical modifications. This includes drug affinity responsive target stability (DARTS), stability of proteins from rates of oxidation (SPROX), cellular thermal shift assay (CETSA), thermal proteome profiling (TPP) and many others. Here we review the principles and applications of these label-free techniques, their advantages and limitations, as well as the most recent advances.