13C代谢流量分析发展30年

Thirty years development of 13C metabolic flux analysis: a review

摘要:

13C代谢流量分析 (13C metabolic flux analysis，13C-MFA)，是通过标记实验分析蛋白氨基酸或胞内代谢物同位素标记异构体的分布情况，从而准确定量胞内反应速率。该技术在系统理解细胞代谢特性、指导代谢工程改造和揭示病理生理学等方面起着重要作用，引起研究者的广泛重视。文中重点综述了代谢流分析30年的发展历程，尤其在工业生物技术和生物医药领域的应用，并对未来的发展方向进行展望。

Abstract:

13C metabolic flux analysis (13C-MFA) enables the precise quantification of intracellular metabolic reaction rates by analyzing the distribution of mass isotopomers of proteinogenic amino acids or intracellular metabolites through 13C labeling experiments. 13C-MFA has received much attention as it can help systematically understand cellular metabolic characteristics, guide metabolic engineering design and gain mechanistic insights into pathophysiology. This article reviews the advances of 13C-MFA in the past 30 years and discusses its potential and future perspective, with a focus on its application in industrial biotechnology and biomedicine.