非传统酵母代谢工程研究进展

Advances in metabolic engineering of non-conventional yeasts

摘要:

近30年来解脂耶氏酵母、克鲁维酵母、毕赤酵母、假丝酵母、汉逊酵母等非传统酵母因其具有天然的生理代谢优势，如快速生长、多底物利用、胁迫耐受性等，在代谢工程领域得到了广泛关注，多种基因工程改造工具正逐渐被开发用于非传统酵母的特性拓展，使其成为合成重组蛋白、生物可再生化学物质的高效细胞工厂。文中总结了非传统酵母中基因编辑工具的发展，并从代谢工程改造策略角度概括了利用非传统酵母进行产品合成的研究进展。最后，讨论了非传统酵母在产品生产应用方面遇到的挑战和未来的研究方向。

Abstract:

Over the past 30 years, Yarrowia lipolytica, Kluyveromyces, Pichia, Candida, Hansenula and other non-conventional yeasts have attracted wide attention because of their desirable phenotypes, such as rapid growth, capability of utilizing multiple substrates, and stress tolerance. A variety of synthetic biology tools are being developed for exploitation of their unique phenotypes, making them potential cell factories for the production of recombinant proteins and renewable bio-based chemicals. This review summarizes the gene editing tools and the metabolic engineering strategies recently developed for non-conventional yeasts. Moreover, the challenges and future perspectives for developing non-conventional yeasts into efficient cell factories for the production of useful products through metabolic engineering are discussed.