基于SH2超亲体的微量样品酪氨酸磷酸化蛋白质组学技术研究进展及应用

Advances and application of enrichment technology in SH2 superbinder-based tyrosine phosphoproteomics

摘要:

酪氨酸磷酸化是生物体内一种重要的蛋白质磷酸化修饰类型，参与细胞信号转导、细胞迁移、凋亡等众多的生命活动过程。在磷酸化蛋白质组学研究中，由于酪氨酸磷酸化蛋白丰度低且有时起始样品量有限，传统的磷酸化蛋白质组富集方法用于酪氨酸磷酸化肽富集效率较低。微量样品的制备技术以及SH2超亲体的引入正在改变酪氨酸磷酸化蛋白质组研究的现状，现对此进行综述。

关键词:酪氨酸磷酸化，SH2超亲体，微量样品，膜蛋白，富集技术

Abstract:

Tyrosine phosphorylation is one of the important protein phosphorylations in eukaryotes responsible for a variety of biological processes including cell signaling transduction, cell migration, and apoptosis. In the study of phosphoproteomics, due to the low stoichiometry of tyrosine phosphorylation (pTyr) proteins and sometimes limited initial sample, traditional phosphoproteomics enrichment technology is inefficient for the enrichment of pTyr peptides. Here, we review the substantial progress in tyrosine phosphoproteomics by preparation of limited amount sample and the newly introduced SH2 superbinder.

Key words:tyrosine phosphorylation, SH2 superbinder, micro-sample, membrane protein, enrichment technology