组织工程细胞片的构建

Construction of tissue engineered cell sheet

摘要:

作为一种无支架的组织工程技术，组织工程细胞片不仅可以避免支架材料带来的不利影响，而且可通过组装进一步形成更为复杂的三维功能化组织，因而在生物医学领域备受关注。细胞片的构建主要是基于敏感性材料所构建的培养基底，通过改变温度、酶、光、离子、氧化还原pH、糖等刺激因素，调节基底对细胞的粘附行为使细胞发生自然脱附，从而获取细胞片。近年来，随着研究的深入进行，特别是各种新型的敏感性培养基底的不断发展，各种简单高效的细胞片构建技术不断涌现，得到的各种具有优良性能的细胞片极大地扩展了其应用的广度和深度。文中对组织工程细胞片的各种构建方法进行了阐述，对其存在的问题及发展前景进行了分析和展望。

Abstract:

Scaffold-free tissue engineered cell sheet is an emerging technology in biomedical field. It can avoid the adverse effects of scaffold materials, and can be further assembled to form more complex three-dimensional functional tissues. The construction of cell sheet is mainly based on the culture substrate composed of sensitive materials. By changing the stimulation factors such as temperature, enzyme, light, ion, redox, pH and sugar, the adhesion behavior of the substrate to the cells could be changed to make the cells detach naturally, thus generating the cell sheet. Recent years have seen the development of various simple and efficient construction technologies of cell sheet due to the development of a variety of novel sensitive culture substrates. The resulted cell sheets with excellent performance have greatly expanded their applications. This review summarized the construction methods of tissue engineered cell sheet and discussed the challenges and future perspectives in this field.