脱细胞基质生物墨水制备方法及应用进展

Preparation and application of decellularized extracellular matrix bioink: a review

摘要:

组织器官脱细胞后制备成的脱细胞基质 (Decellularized extracellular matrix，dECM) 含有许多蛋白质和生长因子，不仅能够为细胞提供三维支架还能够调控细胞再生，是目前最具有生物结构的生物材料。3D生物打印可以层层打印dECM和自体细胞的组合，构建载细胞组织结构。文中综述了不同来源的组织器官脱细胞基质生物墨水制备方法，包括脱细胞、交联等，以及脱细胞基质生物墨水在生物打印中的应用，并展望了其未来的应用前景。

关键词:3D生物打印，组织工程，生物墨水，脱细胞基质

Abstract:

Decellularized extracellular matrix (dECM), which contains many proteins and growth factors, can provide three-dimensional scaffolds for cells and regulate cell regeneration. 3D bioprinting can print the combination of dECM and autologous cells layer by layer to construct the tissue structure of carrier cells. In this paper, the preparation methods of tissue and organ dECM bioink from different sources, including decellularization, crosslinking, and the application of dECM bioink in bioprinting are reviewed, with future applications prospected.

Key words:3D bioprinting, tissue engineering, bioink, decellularized extracellular matrix