纳米孔测序技术在环境微生物研究中的应用

Application of nanopore sequencing in environmental microbiology research

摘要:

高通量测序技术是研究环境微生物的有效手段，而以纳米孔测序为代表的第三代测序技术以其测序读长长、测序速度快、测序数据实时监控、仪器方便携带、无GC偏好性、无需经过PCR扩增等显著优势有力推动了环境微生物研究的发展。本文对纳米孔测序技术的技术原理和特点进行了简要概述，重点介绍了纳米孔测序技术在环境微生物扩增子测序、宏基因组测序、全基因组测序等领域的研究应用，并分析了纳米孔测序技术在环境微生物应用中的优势及存在的问题。

关键词:纳米孔测序技术;扩增子测序;宏基因组测序;全基因组测序;环境微生物

Abstract:

The development of high-throughput sequencing techniques enabled a deeper and more comprehensive understanding of environmental microbiology. Specifically, the third-generation sequencing techniques represented by nanopore sequencing have greatly promoted the development of environmental microbiology research due to its advantages such as long sequencing reads, fast sequencing speed, real-time monitoring of sequencing data, and convenient machine carrying, as well as no GC bias and no PCR amplification requirement. This review briefly summarized the technical principle and characteristics of nanopore sequencing, followed by discussing the application of nanopore sequencing techniques in the amplicon sequencing, metagenome sequencing and whole genome sequencing of environmental microorganisms. The advantages and challenges of nanopore sequencing in the application of environmental microbiology research were also analyzed.

Key words:nanopore sequencing technique;amplicon sequencing;metagenome;whole genome sequencing;environmental microbiology