解脂耶氏酵母表达调控工具的开发及天然产物合成的研究进展

The recent advances in developing gene editing and expression tools and the synthesis of natural products in Yarrowia lipolytica

摘要:

解脂耶氏酵母是一种重要的产油酵母，由于其能利用多种疏水性底物，具有良好的耐酸、耐盐等胁迫耐受性，具有高通量的三羧酸循环，可提供充足的乙酰辅酶A前体等特点，被认为是生产萜类、聚酮类和黄酮类等天然产物的理想宿主，在代谢工程领域有着广泛的应用。近年来，越来越多的基因编辑、表达和调控工具被逐渐开发，这促进了解脂耶氏酵母合成各种天然产物的研究。文中综述了近年来解脂耶氏酵母中基因表达和天然产物合成方面的研究进展，并探讨了在该酵母中异源合成天然产物所面临的挑战和可能的解决方案。

Abstract:

Yarrowia lipolytica, as an important oleaginous yeast, has been widely used in metabolic engineering. Y. lipolytica is considered as an ideal host for the production of natural products such as terpenes, polyketides and flavonoids, due to its ability to utilize a variety of hydrophobic substrates, high stress tolerance to acid and salt, high flux of tricarboxylic acid cycle and the ability in providing abundant the common precursor acetyl-CoA. Recently, more and more tools for genetic editing, gene expression and regulation has been developed in Y. lipolytica, which facilitate the metabolic engineering of Y. lipolytica for bio-manufacturing. In this review, we summarized the recent progresses in developing gene expression and natural product synthesis in Y. lipolytica, and also discussed the challenges and possible solutions in heterologous synthesis of natural products in this yeast.