基于糖原代谢调控的蓝细菌光合细胞工厂优化研究进展

Engineering the glycogen metabolism in cyanobacterial photosynthetic cell factories: a review

摘要:

蓝细菌是重要的光合自养微生物，也是最具潜力的光合微生物底盘之一，被广泛应用于光驱固碳细胞工厂的开发。糖原是蓝细菌最重要的天然碳汇物质，糖原代谢对蓝细菌光合碳流的分配和调控具有重要意义。为了优化蓝细菌光合细胞工厂的合成效能，驱动更多的光合碳流重定向至目标代谢产物的合成，已经有多种策略和方法被成功开发用于调控蓝细菌的糖原代谢和糖原含量。然而，作为具有全局效应的重要碳汇机制，针对糖原代谢的调控往往对蓝细菌底盘藻株的光合生理和代谢网络造成复杂的影响，在不同光合细胞工厂合成效能优化上取得的效果也不尽相同。文中梳理了蓝细菌糖原代谢工程的最新进展，对糖原代谢调控造成的生理、代谢影响进行了介绍和分析，进而对通过糖原代谢调控来优化光合细胞工厂效能的研究前景进行了展望。

关键词:蓝细菌;光合细胞工厂;糖原;代谢工程;光合作用

Abstract:

Cyanobacteria are important photosynthetic autotrophic microorganisms and are considered as one of the most promising microbial chassises for photosynthetic cell factories. Glycogen is the most important natural carbon sink of cyanobacteria, playing important roles in regulating its intracellular carbon distributions. In order to optimize the performances of cyanobacterial photosynthetic cell factories and drive more photosynthetic carbon flow toward the synthesis of desired metabolites, many strategies and approaches have been developed to manipulate the glycogen metabolism in cyanobacteria. However, the disturbances on glycogen metabolism usually cause complex effects on the physiology and metabolism of cyanobacterial cells. Moreover, the effects on synthesis efficiencies of different photosynthetic cell factories usually differ. In this manuscript, we summarized the recent progress on engineering cyanobacterial glycogen metabolism, analyzed and compared the physiological and metabolism effects caused by engineering glycogen metabolism in different cyanobacteria species, and prospected the future trends of this strategy on optimizing cyanobacterial photosynthetic cell factories.

Key words:cyanobacteria;photosynthetic cell factory;glycogen;metabolic engineering;photosynthesis