纳米抗体技术应用的最新进展

Recent advances in the application of nanobody technology:a review

摘要:

纳米抗体作为一种可塑性强、较为新颖的抗原识别和调控的工具，具备小尺寸、易表达和筛选及改造、高亲性和稳定性等优势，能够识别传统抗体难以识别的较为隐匿的抗原表位，在诊断治疗各种疾病及检测方面的应用不断深入，且在基础研究中也发挥着不可替代的作用。文中主要介绍了纳米抗体及其衍生结构在小分子化合物及病原微生物检测和疾病的诊断，以及在疾病靶向治疗，细胞、分子成像领域的相关进展，此外还综述了在蛋白质构象研究领域展现的广阔前景。

Abstract:

As a malleable and novel tool for antigen recognition and modulation,nanobodies have the advantages of small size,easiness of expression,screening and modification,as well as high affinity and stability.Nanobodies are capable of recognizing more cryptic antigenic epitopes that are difficult to be recognized by traditional antibodies,making them increasingly used in the diagnosis and treatment of various diseases and assays.Nanobodies are also playing an irreplaceable role in the basic research.This review summarized the recent development of nanobodies and their derivatives in the detection of small molecules,pathogenic microorganisms and diagnosis of diseases,as well as in the fields of targeted therapies,cellular and molecular imaging.Broad prospects of nanobodies in the field of protein conformation studies were also reviewed.