相容溶质四氢嘧啶的微生物合成研究进展

Advances in the microbial production of the compatible solute ectoine:a review

摘要:

四氢嘧啶(ectoine)作为一种氨基酸的衍生物，是嗜盐微生物胞内重要的天然次级代谢物，具有保护细胞和稳定生物大分子的功能，可广泛应用于药物制备佐剂、器官移植与保存、皮肤创伤修复与新型化妆品研发等生物医学领域。由于四氢嘧啶的医用价值和商业市场需求，文中从野生菌株的筛选与诱变育种、构建基因工程菌株与系统代谢整合工程菌株、四氢嘧啶的优化发酵与生产工艺以及抽提纯化工艺等方面，系统论述了四氢嘧啶高效积聚和过量化生产研究策略，并展望多组学、计算生物学及“细胞工厂”等技术在后续四氢嘧啶高效生产上的应用前景。

Abstract:

Ectoine is an amino acid derivative and an important natural product in halophilic microorganisms.It plays an important role in protecting cells and stabilizing biological macromolecules,and can be widely used in biomedical fields such as drug preparation adjuvants,organ transplantation and preservation,skin wound repair and cosmetics.Due to the medical value and commercial market demand of ectoine,this article summarized the recent advances in the microbial production of ectoine,including the mutation and breeding of hyper-producing strains,construction of genetically and metabolically engineered strains,optimization of fermentation processes,and extraction and purification processes.The application of multi-omics technologies and computational biology to develop an ectoine producing cell factory was prospected,with the aim to provide a reference for ectoine overproduction.