代谢工程改造解脂耶氏酵母合成羧酸的研究进展

Production of carboxylic acids by metabolically engineered Yarrowia lipolytica:a review

摘要:

解脂耶氏酵母是一种具有独特生理代谢特征的非常规酵母。它具有可以利用多种廉价碳源、低pH值耐受性好、分泌能力强等优点,因此非常适合用于各种工业产品的微生物发酵。目前,解脂耶氏酵母已被证实具有高效生产多种(同源或异源)有机羧酸的能力。本文对近年来利用代谢工程及合成生物学技术改造解脂耶氏酵母生产羧酸的实例进行了总结,并重点介绍了所用的代谢工程策略。最后,本文讨论了目前使用解脂耶氏酵母生产羧酸的瓶颈及解决方法,期望为后续相关研究提供有价值的参考。

Abstract:

Yarrowia lipolytica is a non-conventional yeast with unique physiological and metabolic characteristics.It is suitable for production of various products due to its natural ability to utilize a variety of inexpensive carbon sources,excellent tolerance to low pH,and strong ability to secrete metabolites.Currently,Y.lipolytica has been demonstrated to produce a wide range of carboxylic acids with high efficiency.This article summarized the progress in engineering Y.lipolytica to produce various carboxylic acids by using metabolic engineering and synthetic biology approaches.The current bottlenecks and solutions for high-level production of carboxylic acids by engineered Y.lipolytica were also discussed,with the aim to provide useful information for relevant studies in this field.