耐受性工程调控微生物细胞工厂胁迫抗性

Tolerance engineering regulates stress resistance of microbial cell factory

摘要:

微生物细胞工厂的生产效率是由菌株生长性能、产品合成能力和胁迫抗性共同决定的,其中增强微生物细胞工厂的胁迫抗性是关键。耐受性工程基于微生物细胞工厂抵御胁迫压力的应激反应机制,通过巩固壁膜屏障增强胁迫防御能力,加快应激反应提高损伤修复能力,创制耐受进化工具筛选鲁棒性增强的工业微生物。文中分析归纳了耐受性工程的调控策略,并展望了耐受性工程精深调控胁迫抗性的发展前景,对于微生物细胞工厂可持续生产天然产物和大宗化学品具有重要作用。

Abstract:

The production efficiency of microbial cell factory is determined by the growth performance,product synthetic capacity,and stress resistance of the strain.Strengthening the stress resistance is the key point to improve the production efficiency of microbial cell factory.Tolerance engineering is based on the response mechanism of microbial cell factory to resist stress.Specifically,it consolidates the cell wall-cell membrane barrier to enhance the defense against stress,accelerates the stress response to improve the damage repair,and creates tolerance evolutionary tools to screen industrial microorganisms with enhanced robustness.We summarize the regulation strategies and forecast the prospects of tolerance engineering,which plays an important role in the microbial cell factories for sustainable production of natural products and bulk chemicals.