麦角硫因生物合成研究的新进展

Recent progress in ergothioneine biosynthesis:a review

摘要:

麦角硫因是一种独特的细胞生理保护剂,在食品、饮料、化妆品和医药等行业具有广阔的应用前景。生物合成法是麦角硫因制备方法的研究热点。文中介绍了近年来在麦角硫因生物合成途径的鉴定、生物合成关键酶的挖掘、天然可食用菌种以及高产工程菌的开发等方面取得的新进展,以期从分子层面深入认识麦角硫因生物合成的调控机制,进而利用发酵工程、代谢工程和合成生物学的新技术大幅度提升麦角硫因的生物合成水平。

Abstract:

Ergothioneine is a multifunctional physiological cytoprotector,with broad application in foods,beverage,medicine,cosmetics and so on.Biosynthesis is an increasingly favored method in the production of ergothioneine.This paper summarizes the new progress in the identification of key pathways,the mining of key enzymes,and the development of natural edible mushroom species and high-yield engineering strains for ergothioneine biosynthesis in recent years.Through this review,we aim to reveal the molecular mechanism of ergothioneine biosynthesis and then employ the methods of fermentation engineering,metabolic engineering,and synthetic biology to greatly increase the yield of ergothioneine.