微生物模块化共培养工程的应用及控制策略

Application and population control strategy of microbial modular co-culture engineering

摘要:

传统的微生物合成方法通常依赖于单一工程菌株,通过代谢工程改造合成目标产物。由于关键的辅因子、前体和能量被引入到复杂的化合物合成途径中,加重了工程菌株的代谢负担,因而常常会影响目标产物产量。而模块化共培养工程已经成为一种有效进行异源生物合成并有望大大提高产物产量的新方法。在模块化共培养工程中,不同模块菌群之间的相互协调对于生产至关重要。文中重点介绍了模块化共培养工程的应用及种群控制策略。

Abstract:

Traditional methods of microbial synthesis usually rely on a single engineered strain to synthesize the target product through metabolic engineering.The key cofactors,precursors and energy are produced by the introduced complex synthetic pathways.This would increase the physiological burden of engineering strains,resulting in a decrease in the yield of target products.The modular co-culture engineering has become an attractive solution for effective heterologous biosynthesis,where product yield can be greatly improved.In the modular co-culture engineering,the coordination between the population of different modules is essential for increasing the production efficiency.This article summarized recent advances in the application of modular co-culture engineering and population control strategies.