口腔静态生物膜模型研究进展

Advance of in vitro oral static biofilm model

摘要:

口腔静态生物膜模型是体外模拟口腔微生态环境的重要手段，因其成本低、通量高、可靠性好、操作容易等优点，已成为研究各种口腔疾病的发病机制，测试各种药物、口腔护理用品、食品的重要工具。建立口腔静态生物膜模型，可根据研究目的，选择不同的装置、接种源、培养基、基质和培养条件，并通过测定生物量、代谢活性、群落结构以及进行可视化分析等多种方法评价生物膜的生长情况。本文汇总了近年来报道的口腔静态生物膜模型建立和评价的方法学要素，并分析讨论了各种方法的适用范围，希望有助于相关领域研究和生产实践的开展。

关键词:口腔;生物膜;静态模型;阿姆斯特丹主动附着模型;卡尔加里装置;微观世界模型;多物种模型

Abstract:

Oral static biofilm model is an important tool for in vitro simulation of oral microecological environment, which has become an important method for studying the pathogenesis of various oral diseases and testing the efficacy of various drugs, oral care products and foods due to its low cost, high throughput, good reliability and easy operation. The establishment of oral static biofilm models allows the selection of different devices, inoculum sources, media, substrates and culture conditions according to the purpose of the study, and the evaluation of biofilm growth by various methods such as measuring biomass, metabolic activity, community structure and performing visualization analysis. This paper summarizes the methodological elements reported in recent years for the establishment and evaluation of oral static biofilm models, and analyzes and discusses the applicability of various methods in the hope of contributing to the research and production practice in related fields.

Key words:oral;biofilm;static model;Amsterdam active attachment model;Calgary biofilm device;microcosms models;multispecies biofilm models