科微学术

生物工程学报

2025年4月3日 19:55 星期四
首页 > 过刊浏览>2022年第38卷第9期 >3329-3343. DOI:10.13345/j.cjb.210925
PDF HTML阅读 XML下载 导出引用 引用提醒
鸡干扰素γ与白介素2对外周血中Th1细胞分化相关细胞因子的影响
作者:
基金项目:

国家自然科学基金（32070164）；中国科学院B类先导专项（XDB29010000）


Effects of chicken interferon-γ and interleukin-2 on cytokines related to Th1 cell differentiation in peripheral blood
Author:
  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [28]
    • |
  • 相似文献 [20]
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    为了探讨鸡干扰素γ(chicken interferon-γ,ChIFN-γ)与白介素2(chicken interleukin-2,ChIL-2)对1型辅助性T细胞(type 1 helper T lymphocytes,Th1)分化的影响，本研究原核表达、纯化了ChIFN-γ与ChIL-2，并以不同浓度刺激刀豆球蛋白A (concanavalin A,Con A)活化的鸡外周血淋巴细胞，检测其对Th1细胞分化相关细胞因子表达的影响。结果表明，不同浓度的ChIFN-γ与ChIL-2均可上调Th1细胞分化相关细胞因子的转录水平，最佳刺激浓度分别为12.5μg/mL和25.0 μg/mL。然后将ChIFN-γ和ChIL-2分别与H9N2灭活疫苗联用，以口服或肌肉注射方式免疫无特定病原体(specific pathogen free,SPF)鸡，检测其对Th1细胞分化相关细胞因子表达的影响。结果表明，与单独使用H9N2疫苗相比，ChIFN-γ和ChIL-2均能显著上调H9N2疫苗诱导的Th1细胞分化相关细胞因子的转录水平，且肌肉注射的效果优于口服。本研究通过对Th1细胞分化相关细胞因子进行检测，从体外和体内水平证实了ChIFN-γ与ChIL-2能够增强ConA或H9N2灭活疫苗诱导的Th1细胞分化，为其作为疫苗佐剂使用提供了理论依据。

    Abstract:

    This paper aims to explore the effects of chicken interferon-γ (ChIFN-γ) and interleukin-2 (ChIL-2) on type 1 helper (Th1) T lymphocyte differentiation. To be specific, ChIFN-γ and ChIL-2 were first expressed in Escherichia coli competent cells and then purified by Ni-NTA affinity chromatography. Different concentration of ChIFN-γ and ChIL-2 were employed to stimulate the lymphocytes in chicken peripheral blood which had been activated by concanavalin A (Con A), and the mRNA levels of cytokines related to Th1 cell differentiation were detected by real-time quantitative PCR (RT-qPCR). The results showed that both ChIFN-γ and ChIL-2 can significantly up-regulate mRNA levels of cytokines related to Th1 cell differentiation and the optimal concentration was 12.5 μg/mL and 25.0 μg/mL, respectively. In addition, specific-pathogen-free (SPF) chickens were immunized with ChIL-2 or ChIFN-γ together with H9N2 vaccine, or H9N2 vaccine alone by oral administration or intramuscular injection, respectively. The mRNA levels of cytokines related to Th1 cell differentiation were detected after immunization. The results showed that ChIFN-γ and ChIL-2 significantly up-regulated the mRNA levels of cytokines related to Th1 cell differentiation induced by H9N2 vaccine compared with H9N2 vaccine alone, and that the intramuscular injection was better than oral administration. In this study, we verified that ChIFN-γ and ChIL-2 can significantly enhance mRNA levels of cytokines related to Th1 cell differentiation induced by ConA or H9N2 vaccine in vitro and in vivo. The results of this study can lay a theoretical basis for using ChIFN-γ and ChIL-2 as vaccine adjuvants.

    参考文献
    [1] El Zowalaty ME, Bustin SA, Husseiny MI, et al. Avian influenza:virology, diagnosis and surveillance. Future Microbiol, 2013, 8(9):1209-1227.
    [2] Hilton LS, Bean AGD, Lowenthal JW. The emerging role of avian cytokines as immunotherapeutics and vaccine adjuvants. Vet Immunol Immunopathol, 2002, 85(3/4):119-128.
    [3] Pestka S, Langer JA, Zoon KC, et al. Interferons and their actions. Annu Rev Biochem, 1987, 56:727-777.
    [4] Kak G, Raza M, Tiwari BK. Interferon-gamma (IFN-γ):exploring its implications in infectious diseases. Biomol Concepts, 2018, 9(1):64-79.
    [5] Xu G, Zhang P, Dang RJ, et al. Dynamic changes of Th1 cytokines and the clinical significance of the IFN-γ/TNF-α ratio in acute brucellosis. Mediators Inflamm, 2019, 2019:5869257.
    [6] Sad S, Marcotte R, Mosmann TR. Cytokine-induced differentiation of precursor mouse CD8+ T cells into cytotoxic CD8+ T cells secreting Th1 or Th2 cytokines. Immunity, 1995, 2(3):271-279.
    [7] Kim CK, Choi YM, Bae E, et al. Reduced NK cell IFN-γ secretion and psychological stress are independently associated with Herpes zoster. PLoS One, 2018, 13(2):e0193299.
    [8] Frucht DM, Fukao T, Bogdan C, et al. IFN-gamma production by antigen-presenting cells:mechanisms emerge. Trends Immunol, 2001, 22(10):556-560.
    [9] Schroder K, Hertzog PJ, Ravasi T, et al. Interferon-gamma:an overview of signals, mechanisms and functions. J Leukoc Biol, 2004, 75(2):163-189.
    [10] Rahman MM, Eo SK. Prospects and challenges of using chicken cytokines in disease prevention. Vaccine, 2012, 30(50):7165-7173.
    [11] Spolski R, Li P, Leonard WJ. Biology and regulation of IL-2:from molecular mechanisms to human therapy. Nat Rev Immunol, 2018, 18(10):648-659.
    [12] Pol JG, Caudana P, Paillet J, et al. Effects of interleukin-2 in immunostimulation and immunosuppression. J Exp Med, 2020, 217(1):e20191247.
    [13] Hilton LS, Bean AGD, Kimpton WG, et al. Interleukin-2 directly induces activation and proliferation of chicken T cells in vivo. J Interferon Cytokine Res, 2002, 22(7):755-763.
    [14] Wu TN, Lin KH, Chang YJ, et al. Avidity of CD1d-ligand-receptor ternary complex contributes to T-helper 1(Th1) polarization and anticancer efficacy. PNAS, 2011, 108(42):17275-17280.
    [15] Guo PJ, Thomas JD, Bruce MP, et al. The chicken TH1 response:potential therapeutic applications of ChIFN-Γ. Dev Comp Immunol, 2013, 41(3):389-396.
    [16] Song XK, Huang XM, Yan RF, et al. Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-γ or IL-2 against coccidiosis in chickens. Exp Parasitol, 2015, 156:19-25.
    [17] Cardenas-Garcia S, Dunwoody RP, Marcano V, et al. Effects of chicken interferon gamma on Newcastle disease virus vaccine immunogenicity. PLoS One, 2016, 11(7):e0159153.
    [18] Yu SF, Jia L, Zhang YN, et al. IL-12 induced the generation of IL-21- and IFN-γ-co-expressing poly-functional CD4+ T cells from human naive CD4+ T cells. Cell Cycle, 2015, 14(21):3362-3372.
    [19] Oestreich KJ, Weinmann AS. Transcriptional mechanisms that regulate T helper 1 cell differentiation. Curr Opin Immunol, 2012, 24(2):191-195.
    [20] Mullen AC, High FA, Hutchins AS, et al. Role of T-bet in commitment of TH1 cells before IL-12-dependent selection. Science, 2001, 292(5523):1907-1910.
    [21] Liao W, Lin JX, Wang L, et al. Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages. Nat Immunol, 2011, 12(6):551-559.
    [22] 李婷, 包细明, 鲜思美, 等. 羊IL-2和OrfV-F1L基因疫苗联合免疫小鼠的免疫应答研究. 中国兽医科学, 2019, 49(7):924-931. Li T, Bao XM, Xian SM, et al. Study on immune response in mice inoculated jointly with IL-2 gene vaccine and OrfV-F1L gene vaccine. Chin Vet Sci, 2019, 49(7):924-931(in Chinese).
    [23] 申燕. 利用鸡IL-2增强NDV-F抗原表位及其作为疫苗的免疫效果的研究[D]. 哈尔滨:黑龙江大学, 2015. Shen Y. Study on using chicken IL-2 to enhance NDV-F epitope and its immune effect as a vaccine[D]. Harbin:Heilongjiang University, 2015(in Chinese).
    [24] Yasuda K, Nakanishi K, Tsutsui H. Interleukin-18 in health and disease. Int J Mol Sci, 2019, 20(3):649.
    [25] 郝飞飞. 鸡球虫病活疫苗基因佐剂IL-2、IL-4实验室效力和安全性研究[D]. 太谷:山西农业大学, 2019. Hao FF. Research on the laboratory efficacy and safety of gene adjuvant IL-2 and IL-4 of chicken coccidiosis live vaccine[D]. Taigu:Shanxi Agricultural University, 2019(in Chinese).
    [26] Kim WH, Chaudhari AA, Lillehoj HS. Involvement of T cell immunity in avian coccidiosis. Front Immunol, 2019, 10:2732.
    [27] Yang Y, Dong M, Hao X, et al. Revisiting cellular immune response to oncogenic Marek's disease virus:the rising of avian T-cell immunity. Cell Mol Life Sci. 2020 Aug; 77(16):3103-3116.
    [28] Qin Y, Tu K, Teng Q, et al. Identification of novel T-cell epitopes on infectious bronchitis virus N protein and development of a multi-epitope vaccine. J Virol, 2021, 95(17):e0066721.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

刘玲,焦鹏涛,王萌,李晶,孙蕾,范文辉,刘文军. 鸡干扰素γ与白介素2对外周血中Th1细胞分化相关细胞因子的影响[J]. 生物工程学报, 2022, 38(9): 3329-3343

复制
分享
文章指标
  • 点击次数:424
  • 下载次数: 917
  • HTML阅读次数: 1070
  • 引用次数: 0
历史
  • 收稿日期:2021-12-15
  • 录用日期:2022-03-07
  • 在线发布日期: 2022-09-24
  • 出版日期: 2022-09-25
文章二维码
您是第5991342位访问者
生物工程学报 ® 2025 版权所有

通信地址：中国科学院微生物研究所    邮编：100101

电话：010-64807509   E-mail：cjb@im.ac.cn

技术支持：北京勤云科技发展有限公司