长链二元酸发酵菌种创制和工艺研究进展

Strain engineering and fermentation technology for production of long-chain dicarboxylic acid: a review

摘要:

长链二元酸作为合成多种高附加值化学品的原料，已广泛应用于化工、农业和医药等领域，目前全球对于长链二元酸的需求呈逐年增长态势。化学法合成长链二元酸对反应条件要求严苛且工艺复杂，而微生物发酵合成在经济性和难易度等方面具有无可比拟的优势。本文综述了长链二元酸的合成方法，包括化学合成法和微生物发酵法，分子工程选育高产菌株的进展以及生物发酵法生产长链二元酸的产业化现状，并就其存在的问题进行了探讨，最后对合成生物学创制长链二元酸高产菌株进行了总结和展望。

Abstract:

Long-chain dicarboxylic acid (DCA), a building block for synthesizing a variety of high value-added chemicals, has been widely used in agriculture, chemical, and pharmaceutical industries. The global demand for DCA is increasing in recent years. Compared with chemical synthesis which requires harsh conditions and complicated processes, fermentative production of DCA has many unparalleled advantages, such as low cost and mild reaction conditions. In this review, we summarized the chemical and microbial synthesis methods for DCA and the commercialization status of the fermentation process. Moreover, the advances of using molecular and metabolic engineering to create high-yielding strains for efficient production of DCA were highlighted. Furthermore, the challenges remaining in the microbial fermentation process were also discussed. Finally, the perspectives for developing high titer DCA producing strains by synthetic biology were proposed.